O-Antigen Description

Serogroup:O172
Serotype H:
Serotype K:
Strain:
Structure:
->3)aLFucNAc(1->4)aDGlc6Ac(1->P-4)aDGlc(1->3)aLFucNAc(1->3)aDGlcNAc(1->
Comment:- Structure [1]
- Contains 0.7 acetyl groups/repeat
- The structure is very similar to that of the Shigella boydii type 13 O-antigen [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
Orf3
ncbi CAZy
- Reference [3]
GT nameDonorAcceptorPosition | Link.NumSimilar to
Orf4

aDGlc(1->

->4)aDGlc

2 | 3
ncbi CAZy
- Reference [3]
GT nameDonorAcceptorPosition | Link.NumSimilar to
Orf5

aDGlc(1->

GT NameDonorAcceptorScore / e-value
O12-O012_05


247 / 1e-80
O123-WfbF


192 / 3e-59
O186-O186_12


176 / 3e-53
O25-WekC


160 / 3e-47
O109-weiM

aDGlc(1->

->3)aL6dTal

105 / 3e-27
O16-WbbK

aDGlc(1->

->3)aLRha

87 / 2e-21
ncbi CAZy
- Reference [3]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbuB

aLFucNAc(1->

->3)aDGlcNAc

4 | 1
GT NameDonorAcceptorScore / e-value
O177-WbuB


692 / 0.0
O25-WbuB


691 / 0.0
O4-WbuB

aLFucNAc(1->

->3)bDGlcNAc

689 / 0.0
O12-O012_09


635 / 0.0
O26-WbuB

aLFucNAc(1->

->3)bDGlcNAc

617 / 0.0
O29-WbuB


544 / 0.0
O15-WbuB


537 / 0.0
O145-WbuB

aLFucNAm(1->

->3)bDGlcNAc

536 / 0.0
O151-WbfG


512 / 0.0
O118-WbfG


512 / 0.0
O108-O108_18


510 / 0.0
O98-WbwW

aLQuiNAc(1->

->3)bDGlcNAc

446 / 1e-158
O123-WbwH


383 / 2e-133
O181-O181_07


381 / 5e-133
O186-O186_16


342 / 1e-117
O163-O163_11


320 / 5e-109
ncbi CAZy
- WbuB linkage was assigned based on the PCR cross reactions between E.coli O4, O12, O25, O26 and O172
- Reference [3]
NMR
1H-NMR5.58 3.58 3.78 3.56 3.97 3.63 3.81 5.04 3.52 3.91 3.92 3.87 3.84 3.84 5.02 4.09 3.88 3.56 3.77 3.80 3.89 1.96 5.00 4.31 3.96 3.93 4.44 1.18 2.01 4.84 4.37 4.07 3.93 4.46 1.20 2.00
Solvent: D2O
Temp: 30C
pD: 8.2
Ref: TSP 0.00 ppm
Comment: 1H-NMR [1], pD=8.2
13C-NMR96.30 72.60 72.40 76.80 72.80 60.50 101.40 72.10 72.80 74.80 72.20 61.20 98.60 54.30 76.60 69.10 73.10 61.40 22.90 174.10 98.40 49.10 77.00 72.00 67.60 16.00 23.20 175.20 98.70 49.80 72.80 71.90 67.80 16.00 22.70 174.40
Solvent: D2O
Temp: 30C
pD: 8.2
Ref: Dioxane 67.40 ppm
Comment: 13C-NMR [1], pD=8.2
Non-Sugar Components
- Acetate - Phosphate
Enzymes
FlippaseMNNKTNELIGGMLFKGLYIFITSLNALLMVKILSPKDLGVWYVFMTLQTLIFTLNNAIIP NIARQYTLGSLSKELNFNCYIFHRSTQKTFIYLILLILIICAIATFTYLSSVLAILESQN KIVLVSWLIIVFSLCLEVYYSSYDCAFNGMGKFKNVNKINFISRACLFLISIGMIAYDID GRNALLYFCIGYFISNLIKRFFIYRLFISNYHNLCFNSESDTESFYKKNEKIILNLSCMS FISSIGGMLIVRGGMLILPYYVSIEEVGKYGLTYQLFEIAFNLLFTASAIKTPSWIFLYK ENKCELKKSYLKIKYVSLIVMAIGGGVISFYGGQILSLFGLHATLLTTNLCLLLTLIFIL QLNHSISGQLLTIQNKIPYAYASLYTGIGVVLLSMIFIPITGFKGALVAIFISQLAYNNW KWPLEARKKIINV
Trembl: Q6JAB4
PolymeraseMYNMSVNKKITKMFVFCEFLCGLYILFYGEYVSDYLNVETNVKHIYIICFLICIVFFHYL FLYSWCILSVLFFRKSKLTTSVNINSIFLLLVIIYFIFLLKTGSGKVYADGGSIGELSSF DKLLFFPIIILKLNFLIYIYAAGCKKKDNIYYLVLFIFLICELYRGVSFSILLIALIEIE KIKRCFRIKCLLISLPLFVLFVNIVYNIKFMVRLGEHYDYLDIFQTLIMLLGRLSIISNV LYNYEHYYSVSNFVEGFGYSAINEFLEKLTPMPSLFGITEKTTEIGKLIFYHSYGRWDSA IAISVLGILSIVPGQLLEICAILIISFVFIQLIINMLDNTEQQNTVAFFFIILTLYQGFW GLLANYVYALFIYLIIIASCNLMMSKSKGRL
Trembl: Q6JAB3
Literature
  • First Author: Landersjö, C. "Structural analysis of the O-antigen polysaccharide from the Shiga toxin-producing Escherichia coli O172". European Journal of Biochemistry. 268(8). (2001), pp 2239-45 PubMed: [11298740]
  • First Author: Feng, L. "Structural and genetic characterization of the Shigella boydii type 13 O antigen". Journal of Bacteriology. 186(2). (2004), pp 383-392 PubMed: [14702307]
  • First Author: Guo H. "Identification of Escherichia coli O172 O-antigen gene cluster and development of a serogroup-specific PCR assay". Journal of Applied Microbiology. 97(1). (2004), pp 181-190 PubMed: [15186455]