O-Antigen Description

Serogroup:O115
Serotype H:18
Serotype K:-
Strain:CCUG11416
Structure:
bLRha(1->4)
         |
  ->3)bDGlcNAc(1->4)aDGalA(1->3)aDMan(1->3)bDGlcNAc(1->
Comment:- Structure [1]
- bLRha O-acetylated in position 2,3,4 and aDGalA O-acetylated in position 2,3
- NMR data is given for O-deacetylated structure
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WegG

bLRha(1->

->4)bDGlcNAc

1 | 4
GT NameDonorAcceptorScore / e-value
O152-WfgB


290 / 7e-97
O105-WbsX


143 / 1e-40
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WegI

bDGlcNAc(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O66-WfaC


89 / 4e-22
O83-O083_04


87 / 2e-21
O32-WfeA


86 / 5e-21
O93-O093_02


84 / 1e-20
O42-WbeX

bDGlcNAc(1->

->3)bDGalf

84 / 2e-20
O28-WbeX

bDGlcNAc(1->

->3)bDGalf

84 / 2e-20
O152-WfgD

bDGlc(1->

->3)bDGlcNAc

83 / 2e-20
O117-WbeA


81 / 2e-19
O107-WclW


80 / 3e-19
O112-WfeI


80 / 2e-19
O169-O169_03


80 / 5e-19
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WegK

GT NameDonorAcceptorScore / e-value
O87-WfgN


88 / 2e-21
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WegL

aDMan(1->

->3)bDGlcNAc

4 | 1
GT NameDonorAcceptorScore / e-value
O34-O034_12


333 / 3e-114
O58-WffE


313 / 4e-106
O141-WfcD


313 / 8e-106
O187-O187_13


301 / 6e-102
O78-WejO


300 / 5e-101
O88-O088_13


296 / 6e-100
O144-O144_04


288 / 2e-96
O99-WejK

DRha(1->

->?)?DGlcNAc ->?)?DGalNAc

193 / 1e-59
O60-O060_12


167 / 3e-50
O169-O169_07


152 / 2e-44
O183-wbaY


146 / 4e-42
O175-O175_06


143 / 6e-41
O9-WbdC

aDMan(1->

->2)aDMan ->3)aDMan

122 / 8e-34
ncbi CAZy
- Reference [2]
NMR
1H-NMR5.20 3.71 4.03 4.34 4.37 5.11 4.12 3.86 3.85 3.63 3.81 4.82 4.18 3.62 3.37 3.37 1.32 4.68 3.60 3.77 3.53 3.39 3.74 3.94 2.01 4.58 3.80 4.08 3.63 3.42 3.73 3.89 2.08
Solvent: D2O
Temp: 50C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [1]
13C-NMR101.64 69.70 70.59 80.36 72.54 175.76 102.56 70.88 80.29 66.18 74.06 61.51 101.93 71.32 73.52 73.01 73.01 17.57 100.56 56.05 81.60 71.83 76.63 61.91 23.39 175.01 102.82 56.47 77.61 76.76 74.95 62.22 23.51 175.24
Solvent: D2O
Temp: 50C
pD:
Ref: Dioxane 67.40 ppm
Comment: 13C-NMR [1]
Non-Sugar Components
- Acetate
Enzymes
FlippaseMIKRNALSNLVGRIWGIISLFIFIPFYISYLGSTGYGYIGFYNTLVALLAFADLGFSAAT TREFARLSGGDEQNEIEKSKILRSYEFLYLSIVIIVVLTISFGANWISHKWLVASTNVDV SQLIILMALSFGLQLPTNLYVGALMGSERQILANGLQIGWGIFRSAGVLPILHYISNSLE AFFIWQFLSNFLFLIIIYKLTWRKIKSSDKKFSLNILKSTYRYALGMAGMGILASVATQI DKLIVTKNFNIEVVGFYTLAGTLSLIPLIFITTLAKAAFPRFTILKEKQEIEHLKTLYFS LSKIAVVAILPISTVLAIYSHSIIYMWTHSLIIADKASDIVFFLMLAQAIQALTVLPFYL SLSYAYVRINMIFSAIMIIEIPIVYYFAMQHEGTVGVAKSVLITVLTIFIPYMYLLHTRL ISGIFSRWCSEVSISVFLCVIAIYILDRLGVNDRDNLLFTVINGLVTWLILSIIILFGVG IKKP
Trembl: C9EGX8
Polymerase MVLIILLSISMIIAFLIGIRQKDVCCYLIVCMSSFLFAFVVMCIVFLLLKFNGNAELIFPDEMLYVNDST SQLFFSRYVQFFDKNFSLDVVRWINITLFNISLAFLASEISSYLYGRTRLFCSLLAITGTIVGGYWAFFI LKEAFSVAALSMLIVSYIRKSKVFLVFASCLLFFARPELLLLYIAITIAFYFKRKNTHLYYLAIVTSMVC FILFMNSEYSYSIKLFTLSRRFGESEFEFDDVAITTSNLSFFPFIMSEPFRQAVMTNINSTFNPFLDLNP LVVVQRLYNIIGFYIFLTCVKPYLMKDKLYSFAFIVLLGVLFTHSVYRYFNTILIPFSLYFLFLKYRGLN KGSYK
Trembl: E2DNL0
Literature
  • First Author: Fontana C. "Structural studies of the O-antigen polysaccharide from Escherichia coli O115 and biosynthetic aspects thereof". Glycobiology. 23(3). (2013), pp 354-362 PubMed: [23193180]
  • First Author: Wang Q. "Development of a serogroup-specific multiplex PCR assay to detect a set of Escherichia coli serogroups based on the identification of their O-antigen gene clusters". Molecular and Cellular Probes. 24(5). (2010), pp 286-90 PubMed: [20561581]