O-Antigen Description

Serogroup:	O146
Serotype H:
Serotype K:
Strain:
Structure:	aDGlc3SLac(1->3) \| ->2)aLRha(1->2)aLRha(1->4)bDGal(1->3)bDGalNAc(1->
Comment:	- Genetic information [1] - Structure [2]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbwU

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbwV

aLRha(1->

->4)bDGal

GT Name	Donor	Acceptor	Score / e-value
O54-O054_10			214 / 6e-69
O69-O069_11			162 / 1e-48
O133-O133_07	aLRha(1->	->2)aLRha ->4)aDGalA	160 / 6e-48
O147-WfbY			156 / 9e-47
O71-WdaM	aLRha(1->	->4)bDGal	155 / 1e-46
O120-O120_14			151 / 9e-45
O19-O019_08			147 / 4e-43
O35-WcnY			140 / 1e-40
O129-RfbF			134 / 2e-38
O135-RfbF			134 / 2e-38
O13-RfbF			134 / 2e-38
O19-O019_09			133 / 5e-38
O148-WbbQ			128 / 5e-36
O150-WfgI			122 / 7e-34
O10-O010_09			112 / 3e-30
O2-wegQ	aLRha(1->	->3)aLRha ->2)aLRha	101 / 2e-26
O50-O050_12			101 / 2e-26
O177-WbdY			94 / 5e-24
O148-WbbR			84 / 1e-20

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbwW

bDGal(1->

->3)bDGalNAc

GT Name	Donor	Acceptor	Score / e-value
O185-O185_03			279 / 9e-95
O174-WbcZ			273 / 3e-92
O178-O178_09			272 / 6e-92
O153-O153_06			272 / 6e-92
O71-WdaK	bDGal(1->	->3)aDGalNAc	258 / 9e-87
O112-WfeJ			257 / 3e-86
O104-WbwC	bDGal(1->	->3)bDGalNAc	256 / 5e-86
O171-O171_13			253 / 2e-84
O160-O160_08			248 / 8e-83
O155-WclG			237 / 3e-78
O37-O037_13			235 / 1e-77
O5-O05_15			231 / 4e-76
O33-O033_09			224 / 2e-73
O70-O070_13			218 / 5e-71
O131-O131_11			208 / 3e-67
O117-WbeD	bDGal(1->	->3)aDGalNAc	191 / 2e-60
O107-WclZ	bDGal(1->	->3)aDGalNAc	190 / 4e-60
O7-WbbD	bDGal(1->	->3)aDGlcNAc	172 / 2e-53
O65-O065_13			161 / 4e-49
O28-O028ab_02			115 / 6e-32
O64-O064_03			108 / 2e-29
O54-O054_06			107 / 5e-29
O63-WbuP			94 / 2e-24
O74-O074_09			88 / 3e-22
O55-WbgO			87 / 1e-21
O55-WbgO			87 / 1e-21
O127-WbiP			86 / 1e-21
O90-O090_13			86 / 1e-21
O92-O092_07			85 / 5e-21
O114-WbuP	bDGal(1->	->3)aDGlcNAc	84 / 5e-21
O86-WbnJ	bDGal(1->	->3)aDGalNAc	81 / 1e-19
O23-O023_06			81 / 6e-20
O128-WbsK	bDGalNAc(1->	->4)aDGal	81 / 1e-19
O113-WbnA			81 / 8e-20
O86-WcmC	bDGal(1->	->3)aDGalNAc	80 / 1e-19

ncbi CAZy

- Reference [1]

NMR
1H-NMR		4.81 4.08 3.73 4.13 3.67 3.77 3.80 4.43 3.58 3.74 3.99 3.72 3.72 3.77 5.34 4.12 3.90 3.46 3.71 1.28 5.11 4.45 3.94 3.38 3.73 1.28 4.44 1.46
Solvent:	D2O
Temp:	40C
pD:
Ref:	TSP 0 ppm
Comment:	1H-NMR [2]
13C-NMR		103.0 52.7 81.8 69.3 76.1 62.1 106.4 71.7 74.3 77.2 76.3 62.6 101.4 80.0 71.0 73.2 70.8 17.8 102.2 74.6 74.8 72.0 70.7 18.0 95.5 72.5 83.3 70.0 72.6 61.6 180.8 78.8 20.0
Solvent:	D2O
Temp:	40C
pD:
Ref:	TSP -1.6 ppm
Comment:	13C-NMR [2]

Enzymes
Flippase		MSIKMKANTSNTKRLFFNSIIMYLRMILLMLITLYISRVTINTLGIEKFGLYSVISGFVLLAGVFTNILE STSQRFLSKSLGEGGNVSSIFESCISTHIVLSLIAALLIEMLGVWFINYKLRQDVISINEVYIIFHLSVI TFVVTVITSPFYAALISFENVNYYSIFTLCDVISKVVGISLAPQLPWEITINYAAMILITTLVNRVILTS CFVMRYPKLGCRPSLDFCQMKNILSFAVWNMWGGAATVLNSQGINIVINVFFGILFNSARAITSQINSAI LQLTNSIQLALNPQIVKSYFKDNSCYLNQLIICSGKINVYLTLLIICIFLPNINFLLTVWLGNFPVMTIL LIKLMFIELFINSFSASLSSLIQATGKIKIYQMVVGGVMLLNLPLCLLILSIFKEVEIVYLVAIFVALVC LVLRLIFVNMNSKINIVSYLTNVILPGGGIAFIIYLIQNRWLTEATDLLTALINILYTLFLYSVLIIV
Trembl:	A6M9D5
Polymerase		MIFFLFRQYPLLKYSSIMNVSCLLFLIFVTFFIKRGVFSSGIFLFSLIFLSVHGLYSLYSGNDPVLISRF YIILILIIVSYYAAIKSISYINIFIFLAVTQAFVIIGLEAFMFLNFRFSDYSAIRNYFIDNQFGDIYTYN GFFYHIQIKGNALLLFSYMISFYLYNKTNNKLYLLSSMFLALAVLFCGNLAFYITFLIHAFIFLFLRKAR TYNQLLLKVFIFLITFGVFISYSGMEYLVKAYELKFQGANFSSMGTRFDQFNVLIDDLFENVLTALVGQG LGNIINVQTAVRNYSDYIYYELQAVYFLNQLGVLLFLLFVIINVILTLKFIKPIELRIVYMLYILYALVN PYMLDTNHVVVVFILVSLSSIFSKGGDCSYNGKKHISSYNYI
Trembl:	A6M9D8

Literature
First Author: Liu, Y. "Sequencing and analysis of the Escherichia coli serogroup O117, O126, and O146 O-antigen gene clusters and development of PCR assays targeting serogroup O117-, O126-, and O146-specific DNA sequences". Molecular and Cellular Probes. 21(4). (2007), pp 295-302. PubMed: [17452091]
First Author: Knirel YA. "Structures and gene clusters of the O-specific polysaccharides of the lipopolysaccharides of Escherichia coli O69 and O146 containing glycolactilic acids: ether conjugates of D-GlcNAc and D-Glc with (". Glycoconjugate Journal. (2016),

aLRha(1->

->4)bDGal

aLRha(1->

->2)aLRha ->4)aDGalA

aLRha(1->

->4)bDGal

aLRha(1->

->3)aLRha ->2)aLRha

bDGal(1->

->3)bDGalNAc

bDGal(1->

->3)aDGalNAc

bDGal(1->

->3)bDGalNAc

bDGal(1->

->3)aDGalNAc

bDGal(1->

->3)aDGalNAc

bDGal(1->

->3)aDGlcNAc

bDGal(1->

->3)aDGlcNAc

bDGal(1->

->3)aDGalNAc

bDGalNAc(1->

->4)aDGal

bDGal(1->

->3)aDGalNAc

->2)aLRha
->4)aDGalA

->3)aLRha
->2)aLRha