O-Antigen Description

Serogroup:O96
Serotype H:
Serotype K:
Strain:G3132
Structure:
   ->2)aDGlcA(1->4)bDGlcNAc(1->5)bDGalf(1->3)bDGlcNAc(1->
          |
bDGalf(1->4)
Comment:- Structure [2]
- Strain used for gene sequence determination was H319 [1]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
O096_04

bDGalf(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O96-O096_08


112 / 2e-30
O124-WfeT

bDGalf(1->

->3)bDGalNAc

93 / 1e-23
O164-WfeT


93 / 1e-23
O167-WfdK

bDGalf(1->

->3)bDGlcNAc

91 / 8e-23
O85-WfbU

bDGalf(1->

->3)bDGlcNAc

90 / 2e-22
O170-O170_08


90 / 1e-22
O167-WfdJ


87 / 1e-21
O170-O170_O4


87 / 1e-21
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
O096_06
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
O096_07

bDGlcNAc(1->

GT NameDonorAcceptorScore / e-value
O42-WbeX

bDGlcNAc(1->

->3)bDGalf

120 / 6e-33
O28-WbeX

bDGlcNAc(1->

->3)bDGalf

120 / 8e-33
O112-WfeF


102 / 1e-26
O160-O160_04


94 / 1e-23
O12-O012_O2


86 / 5e-21
O112-WfeM


81 / 3e-19
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
O096_08

bDGalf(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O124-WfeT

bDGalf(1->

->3)bDGalNAc

305 / 7e-104
O164-WfeT


305 / 7e-104
O170-O170_08


278 / 2e-93
O85-WfbU

bDGalf(1->

->3)bDGlcNAc

272 / 4e-91
O167-WfdK

bDGalf(1->

->3)bDGlcNAc

267 / 4e-89
O42-WbeY

bDGalf(1->

->3)aDGlcNAc

213 / 3e-68
O28-WbeY

bDGalf(1->

->3)aDGlcNAc

212 / 7e-68
O170-O170_O4


159 / 1e-47
O96-O096_04


112 / 2e-30
O167-WfdJ


84 / 2e-20
ncbi CAZy
- Reference [1]
NMR
1H-NMR5.70 3.75 3.77 3.67 4.15 4.68 3.71 3.88 3.72 3.58 3.76 3.91 5.02 3.99 4.26 4.14 3.96 3.69 3.69 4.74 3.83 3.63 3.49 3.47 3.76 3.93 5.00 4.08 4.05 4.09 3.82 3.65 3.68 2.04 2.05
Solvent: D2O
Temp: 40C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [2]
13C-NMR99.2 81.1 71.6 79.5 72.4 175.1 102.2 57.1 75.4 77.6 75.4 62.0 109.4 82.4 77.2 82.7 79.0 62.5 103.8 56.4 82.3 69.6 77.0 62.2 109.1 82.5 78.1 84.6 72.1 64.2 23.3 23.4 175.5 176.1
Solvent: D2O
Temp: 40C
pD:
Ref: Acetone 31.45 ppm
Comment: 13C-NMR [2]
Enzymes
FlippaseMKINTTLNKNILYLAVVQGSSYILPLITFPYLVRVLGPELFGVLGFCQASMQYLVLLTDY GFNWTATQQVAKNKNDIVKLTRIFWSVFFAKVFLASISFILLAACCFLIERYKELWFVLF SFSPLVLGNVIYPVWFFQGMEKMKWITICTITARCLVIPLTFIFVKNGQDVWVAALIQGM VNLLAGLLGLCLIKKKRWVNTIIVDYIDIKQCLKDGWHVFISTSAISLYTTSTTVILGFV AGPLAVGYFNVANTIRNAAQGLLTPFTQSIYPRINAVFDSDYLQAIKIIKKSLRYVGGLA FLGSVFLYLLAPFIIKIGVGGGYEESISVLRIMAFLPFIIVLSNIFGVQTMLTHNYKRQF SQILLVSGVANLIIIYPLVIMYSANGAAISLLVTELFVTAQMYLFLRAKKIYLIK
Trembl: A0A0A8J588
PolymeraseMTSSNKILSLLFGVILFLPLFPVQLNETQYHVTLAILLGLILIFLMSWKQLGGFKGGSPV LYYYLCLQLLLILSGVMGVDTVKEYQDLISLFRPMVLLSSFYLLYCLTSRNRDFFRTLIK PMKFICMAMFIWAVYESIIQNNIFQSINYLLYKMERKDDIKNVAVTFFFLPYYSAFVSVF MIAFFYTCRYTYKIRGTYKYIFMSFCCIVLTQSKTGVVAALFLIVCCELISSSQLKRIFC AVCVIILFFISFYFYDYLLMYLNLYFPGNFTRTVSQLADDPSSAYTLSARIEQFLSIYSQ TMQHIPFIGYGLGRDLLIESWPATIYVRYGWIGICVFLIFNFFLIFKGICYYLTHTSNAF RYQAIIFSFWIISTLISQMSAMMTENTKTSFIYLVYLVIFMQLIYKPHKINKSNLG
Trembl: A0A0A8J4S7
Literature
  • First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
  • First Author: Guo X. "Structure and gene cluster of the O-antigen of Escherichia coli O96". Carbohydrate Research. (2015),