O-Antigen Description

Serogroup:O177
Serotype H:
Serotype K:
Strain:
Structure:
->2)aLRha(1->3)aLFucNAc(1->3)aLFucNAc(1->3)bDGlcNAc(1->
Comment:- Structure [3]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbdY

aLRha(1->

GT NameDonorAcceptorScore / e-value
O19-O019_08


130 / 5e-37
O147-WfbY


116 / 8e-32
O2-wegQ

aLRha(1->

->3)aLRha
->2)aLRha

98 / 2e-25
O50-O050_12


98 / 2e-25
O148-WbbQ


97 / 6e-25
O146-WbwV


94 / 5e-24
O69-O069_11


94 / 5e-24
O150-WfgI


93 / 1e-23
O19-O019_09


92 / 2e-23
O35-WcnY


91 / 4e-23
O129-RfbF


89 / 3e-22
O135-RfbF


89 / 3e-22
O13-RfbF


89 / 3e-22
O139-WfaI


81 / 1e-19
O120-O120_14


81 / 2e-19
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbdZ

GT NameDonorAcceptorScore / e-value
O123-WbwH


86 / 1e-20
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbuB

aLFucNAc(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O25-WbuB


706 / 0.0
O4-WbuB

aLFucNAc(1->

->3)bDGlcNAc

703 / 0.0
O172-WbuB

aLFucNAc(1->

->3)aDGlcNAc

692 / 0.0
O12-O012_09


642 / 0.0
O26-WbuB

aLFucNAc(1->

->3)bDGlcNAc

624 / 0.0
O29-WbuB


555 / 0.0
O15-WbuB


549 / 0.0
O145-WbuB

aLFucNAm(1->

->3)bDGlcNAc

548 / 0.0
O151-WbfG


523 / 0.0
O118-WbfG


523 / 0.0
O108-O108_18


518 / 0.0
O98-WbwW

aLQuiNAc(1->

->3)bDGlcNAc

451 / 1e-160
O181-O181_07


383 / 1e-133
O123-WbwH


382 / 5e-133
O186-O186_16


341 / 3e-117
O163-O163_11


323 / 2e-110
ncbi CAZy
- Reference [2]
NMR
1H-NMR5.08 4.00 3.72 3.31 3.56 1.30 5.03 4.37 3.91 3.99 4.35 1.20 2.06 5.01 4.33 3.76 4.02 3.90 1.29 2.04 4.66 3.93 3.69 3.54 3.47 3.75 3.92 2.00
Solvent: D2O
Temp: 50C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [3]
13C-NMR95.78 79.72 70.56 72.90 70.07 17.58 98.69 48.06 73.22 67.80 67.44 16.11 174.85 23.07 94.19 47.92 73.17 67.53 67.84 16.36 174.96 22.89 103.35 56.41 79.57 69.24 76.51 61.58 175.10 23.04
Solvent: D2O
Temp: 50C
pD:
Ref: Dioxane 67.40 ppm
Comment: 13C-NMR [3]
Enzymes
FlippaseMLTKYTLIRNSILNLSGYIIPTLVAIPALGYMARELGPELFGVYTLAMALVGYASVFDFGLTRAIIREIA IYRDDIVEKRKIISTSTVFLTVIGFVVTELIFINVNTIVTFINVSKNNFEDANIALKILSLSIPLLLLNQ LWVSVFEGEEKFGLINIQKTISNTCIVALPAVSILIESSLTYAVSGLVVGRVISLILSFLFLRKEIVASG IRFHTIVMKRLIMFGSWMTFSNIISPMMVYFDRFIISNILGAKNVGFYTAPAEIISRMSIIPTSVSRALF PRLSNMSDFKKFKCELLFSYAIMISICVPIVLFLLLFSGGIMYYWLGPQYYLKSSSVFSVLLIGFFFNSL AQLPFSAIQSLGNSKITALLHGFEIIPYLIILYILTSHFGIVGTAYAWTLRVMFDFIALYFLSSWLIKKK H
Trembl: Q3IA86
PolymeraseMKNKGGNVSLIQRVSSLLIALTIYLFLFDVILLGSGAWSINVTGISIRKVEYLTILLMVICTINNIKGYI YFCCATTFCLIFGVVVPFLNDVKLEYAITELLSFYGILLCPLIAQNRYISVNWKKIRKFILFICIISAFI HILIWLLGLMGSGYVDLIKQLCIRVLTANNDDLIDNIIIADTPDGLFRVLLPNSSLLIIGFYLSFQQYLN RKNYIHLLIVFVMLLALYTTWTRALYLSPIIIIGGVLFYKIFPFTLQLGKIGAYNLLSFIILFFIVISTV LVQPTLLSLLGIASESSDGIRYTQVIWIFNTFMNSPVLGTGLGGSAEDVRSLIAPWTYEMSLLALIMKLG LVGVFLFISISAIKIPEFLEGFFDGRRIPVKKVVWIYFSLSVLMMFSTNPFMLSFPGVTITLFILCELNS FKIEKGNI
Trembl: Q3IA84
Literature
  • First Author: Scheutz, F. "Designation of O174 and O175 to temporary O groups OX3 and OX7, and six new E. coli O groups that include Verocytotoxin-producing E. coli (VTEC): O176, O177, O178, O179, O180 and O181". Acta Pathologica, Microbiologica, et Immunologica Scandinavica. 112(9). (2004), pp 569-84 PubMed: [15601305]
  • First Author: Beutin, L. "Development of PCR assays targeting the genes involved in synthesis and assembly of the new Escherichia coli O 174 and O 177 O antigens". Journal of Clinical Microbiology. 43(10). (2005), pp 5143-5149 PubMed: [16207976]
  • First Author: Svensson MV. "Structural studies of the O-antigenic polysaccharide from Escherichia coli O177". Carbohydrate Research. 346(14. (2011), pp 2300-3 PubMed: [21867992]