O-Antigen Description

Serogroup:	O131
Serotype H:
Serotype K:
Strain:	S239
Structure:	->8)aNeu5Ac(2->6)bDGal(1->6)bDGal(1->3)bDGalNAc(1->
Comment:	- Structure [2] - Strain is for GT data

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O131_08

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O131_10

GT Name	Donor	Acceptor	Score / e-value
O46-O046_07			87 / 8e-22
O134-O134_07			83 / 2e-20

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O131_11

bDGal(1->

->3)bDGalNAc

GT Name	Donor	Acceptor	Score / e-value
O155-WclG			384 / 7e-136
O104-WbwC	bDGal(1->	->3)bDGalNAc	332 / 8e-116
O71-WdaK	bDGal(1->	->3)aDGalNAc	264 / 5e-89
O33-O033_09			263 / 3e-88
O178-O178_09			260 / 2e-87
O153-O153_06			260 / 2e-87
O174-WbcZ			256 / 8e-86
O171-O171_13			254 / 3e-85
O5-O05_15			246 / 8e-82
O146-WbwW			239 / 2e-79
O160-O160_08			239 / 2e-79
O37-O037_13			229 / 2e-75
O117-WbeD	bDGal(1->	->3)aDGalNAc	227 / 9e-75
O107-WclZ	bDGal(1->	->3)aDGalNAc	226 / 3e-74
O112-WfeJ			220 / 5e-72
O185-O185_03			218 / 8e-71
O70-O070_13			207 / 8e-67
O7-WbbD	bDGal(1->	->3)aDGlcNAc	168 / 1e-51
O65-O065_13			151 / 2e-45
O28-O028ab_02			110 / 3e-30
O64-O064_03			109 / 9e-30
O54-O054_06			102 / 4e-27
O114-WbuP	bDGal(1->	->3)aDGlcNAc	89 / 1e-22
O92-O092_07			89 / 2e-22
O63-WbuP			86 / 1e-21
O74-O074_09			82 / 4e-20

ncbi CAZy

- Reference [1]

NMR
1H-NMR		4.41 3.51 3.62 3.92 3.67 3.76 3.76 4.43 3.52 3.61 3.93 3.83 3.88 4.01 4.64 3.95 3.87 4.15 3.66 3.76 3.76 1.82 2.24 3.99 3.88 4.10 3.68 3.92 3.73 3.83 2.02 2.04
Solvent:	D2O
Temp:	40C
pD:
Ref:	TSP 0 ppm
Comment:	1H-NMR [2]
13C-NMR		104.8 72.0 74.1 69.9 76.3 62.2 105.9 71.8 73.6 69.9 74.9 70.5 101.8 53.0 81.2 69.2 75.9 62.2 176.0 97.4 40.6 68.6 53.6 71.1 68.4 79.2 62.2 23.4 23.6 176.1 176.1
Solvent:	D2O
Temp:	40C
pD:
Ref:	TSP -1.6 ppm
Comment:	13C-NMR [2]

Enzymes
Flippase		MSVYKNSSIYLISNIFNALIPFILLPILTRNLTPYEYGQIAMFQTLVSGLASLTGLNTVG AANRRYYDITSKSGLAVYNTNCFYILILSCAALLLLSLLLSDLLSSLLTIPNNWVYLSVV ISGATFIIQFRLGQWQIREKAFWFGILQISQSVIVSVLTIIFLICMHQGASSRVNSLFMV SIVYALVSLYSLYKSKLIMLTKINLLDVRDALYFGVPLIPHILGIFCLSALDRVLINGVL GASEAGIYMLAAQLSLGMMVFFDALNKALVPWLFNALSLNDVNKINSLIRFTYLFFIIVA ILGGLSFWIGPLVVELVAGNEYLNAKNIIGWLCLGQAFNGMYLMVTNYLFYAKCTGRLSI VTIFSGGVNIVLLLVLMKFLGITGVAISFSISMFIRFLATWWLAAKVCGFSWRMSIRRII FG
Trembl:	A0A0A6ZF35
Polymerase		MGFIKVFNLKNSIVDDRYNLLWLFAGLLVVFVLGVLLFPLAGFFLFFFVAFLIANSSKFK LKRMVFFVLYFMLVMCIIINENSIQRFIYREDDFTTYYNNYLELLNGNYEFLFQFGGGAE IGLPALNYIFSFFIGNPFPYFLQMTYIGMYIVMLYYLVSIDRYFGNRDKSNKLDLLLWAT LFLKITAMLTIERQAVASFFILYAISDIRRKYLWLFIGCLFHLSTPVVYLAVRFVLNTKT NKKVLVSCIALILFVVFSHQLLSVINHILPNDKVGYVLYYINNGDFIKNELVKSIKQVSY VIPLLLLDFAMRLQGYRWKLSSSLQLFVYSMLILSFLPGVPTRIFMPIVFILYGFYYYDF ICLFRIKTRVIIFLIITSFFSVYKFFLPGYYYRYPIANIYPGYYISSFFDKYGYVERYSL PYSSDININNDDKL
Trembl:	A0A0A8J5X5

Literature
First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
First Author: Perepelov AV. "Structure elucidation and analysis of biosynthesis genes of the O-antigen of Escherichia coli O131 containing N-acetylneuraminic acid". Carbohydrate Research. 436. (2016), pp 41-44 PubMed: [27863302]