O-Antigen Description

Serogroup:	O133
Serotype H:
Serotype K:
Strain:	N282
Structure:	->3)aLRha(1->2)aLRha(1->2)aLRha(1->4)aDGalA(1->3)aDGlcNAc(1-> \| aDGlc(1->3)
Comment:	- Structure [2] - Strain for structure elucidation was G1272

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O133_07

aLRha(1->

->2)aLRha

->4)aDGalA

1 |

GT Name	Donor	Acceptor	Score / e-value
O146-WbwV			160 / 6e-48
O69-O069_11			157 / 9e-47
O54-O054_10			150 / 2e-44
O147-WfbY			144 / 4e-42
O19-O019_09			143 / 1e-41
O148-WbbQ			141 / 9e-41
O10-O010_09			140 / 2e-40
O129-RfbF			130 / 6e-37
O13-RfbF			130 / 6e-37
O135-RfbF			129 / 1e-36
O19-O019_08			125 / 4e-35
O2-wegQ	aLRha(1->	->3)aLRha ->2)aLRha	122 / 8e-34
O50-O050_12			122 / 8e-34
O120-O120_14			121 / 2e-33
O150-WfgI			114 / 4e-31
O35-WcnY			114 / 3e-31
O71-WdaM	aLRha(1->	->4)bDGal	109 / 2e-29
O148-WbbR			94 / 6e-24

ncbi CAZy

- Reference [1]
- Function inferred in [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O133_08

aDGalA(1->

->3)aDGlcNAc

2 |

GT Name	Donor	Acceptor	Score / e-value
O158-wcmT			362 / 1e-125
O116-O116_11			339 / 7e-117
O100-O100_11			259 / 8e-86
O10-O010_12			245 / 3e-80
O138-WfaH	aDGalNAcA(1->	->3)bDGlcNAc	168 / 1e-50
O121-WbqI			166 / 9e-50
O101-O101_07			164 / 2e-49
O89-O089_07			164 / 5e-49
O103-WbtG			149 / 2e-43
O52-Orf16			130 / 1e-36
O111-WbdH	aDGal(1->	->3)bDGlcNAc	125 / 6e-35
O40-WffO			95 / 5e-24
O113-WbnE			84 / 3e-20
O3-WclR			83 / 7e-20

ncbi CAZy

- Reference [1]
- Function inferred in [2]

NMR
1H-NMR		5.03 4.06 3.99 3.77 4.04 3.79 3.81 5.37 4.10 4.07 4.61 4.22 5.62 4.05 3.86 3.41 3.72 1.25 5.30 4.07 3.88 3.44 3.79 1.31 4.97 4.22 3.83 3.53 3.76 1.29 5.15 3.59 3.64 3.44 3.96 3.76 3.83
Solvent:	D2O
Temp:	30C
pD:
Ref:	TSP 0 ppm
Comment:	1H-NMR [2]
13C-NMR		97.9 72.5 74.2 70.7 73.2 61.8 95.6 53.4 80.9 71.6 72.9 61.4 102.1 68.6 77.7 72.3 72.9 175.0 98.9 76.9 72.0 73.4 70.4 17.8 101.1 80.0 71.0 73.4 70.4 17.9 103.4 68.0 76.5 71.6 70.5 17.9
Solvent:	D2O
Temp:	30C
pD:
Ref:	TSP -1.6 ppm
Comment:	13C-NMR [2]

Enzymes
Flippase		MNKILKDIPIKSILHLFIVRGSIYLFPFITLPIITRNLGVAHFGILSLFLAAQQYFVMLV EYGFTLTGARDIVRCDNKQDEMKIINEIILCRLLIFLFCTAIIIGAYAFFTEHGYISCYL ILWSTVFCSVFNQTHFFIGKEKTGFIVISTCITRIVSIVIVVLYVKDNRDINIALLAYSI NTALPNILSTFYLNLYLKYKVKIIFCNNEIIDRFKSGYDIFISNVFTSIYSTLTLMYLGT AKGSIETGYYSSADKLKAAAQGILFPVAQAFFPRVSKECGISFYLLWRKSTTILVCFAVA IVASLFIFSNLIYILLLGEQYLSGINIYYILTLSIIPISFGISYAQNLYLTQGQTKLLRK IYFAVSVLHLFHMPILVHFLGAIGAAISVLITESFASLLMFIFRKKCYTWSQA
Trembl:	A0A0A8J6K1
Polymerase		MAYLFISLLFVLLFAEIKITQRKFSPTIVLITLWIGVLIVTQIYSSALWQLSVETMWWVF GGVFAFFIGGFWGKLFFKIYGFKNISLQSNGNTLLFAIPFYILMFFFYYFMVRAGANEDQ NWYVGIRKIINYGEPDLFFIMFGYIYYLIYPMLYLEAARYYSSKSFNKKDKYNYHMQLIM CILYAVMSTAKLKLLLVIVPVFFIRSYFKPTSTKLIIVVGTMFLGVFFLSLILLNKIAGD IGVFKIIITSIGNYTFFNLYAFDSLHFKEFLKIKCIDNSNSCSLLPFFEYGDFRTNIYTI MYSFIEYGFFSYSLFLFMVGFIHNAADQIAKKTSNIYAIILSSVLYVPLVFQIMDNQYTA SKYLIYILMFSYVVYFTNVRRLRVM
Trembl:	A0A0A8J8J3

Literature
First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
First Author: Shashkov AS. "Structure and gene cluster of the O-antigen of Escherichia coli O133". Carbohydrate Research. 430. (2016), pp 82-84 PubMed: [27203746]