O-Antigen Description

Serogroup:	O129
Serotype H:
Serotype K:
Strain:	G1096
Structure:	aDGlc(1->3) \| ->2)aLRha3Ac4Ac(1->2)aLRha(1->3)aLRha(1->3)bDGlcNAc(1->
Comment:	- Structure [2] - aLRha is 3,4-O-acetylated in non-stoichiometrical amounts

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

RfbF

aLRha(1->

GT Name	Donor	Acceptor	Score / e-value
O13-RfbF			607 / 0.0
O135-RfbF			606 / 0.0
O150-WfgI			250 / 1e-82
O19-O019_08			191 / 4e-60
O69-O069_11			175 / 9e-54
O19-O019_09			172 / 1e-52
O120-O120_14			167 / 1e-50
O10-O010_09			155 / 5e-46
O35-WcnY			146 / 5e-43
O146-WbwV			134 / 2e-38
O2-wegQ	aLRha(1->	->3)aLRha ->2)aLRha	133 / 5e-38
O50-O050_12			133 / 5e-38
O148-WbbQ			130 / 5e-37
O133-O133_07	aLRha(1->	->2)aLRha ->4)aDGalA	130 / 7e-37
O147-WfbY			125 / 3e-35
O54-O054_10			125 / 3e-35
O71-WdaM	aLRha(1->	->4)bDGal	113 / 1e-30
O139-WfaI			106 / 2e-28
O177-WbdY			89 / 3e-22
O148-WbbR			85 / 5e-21

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

RfbG

->3)bDGlcNAc

GT Name	Donor	Acceptor	Score / e-value
O135-RfbG			617 / 0.0
O13-RfbG	aLRha(1->	->3)bDGlcNAc	617 / 0.0
O154-O154_12			298 / 2e-101
O105-WbsY			290 / 2e-98
O49-O049_13			287 / 5e-97
O150-WfgK			286 / 6e-97
O10-O010_11			107 / 2e-28
O97-O097_05			105 / 6e-28
O154-O154_14			94 / 4e-24
O180-wfcT			91 / 5e-23
O100-O100_10			90 / 2e-22
O18-wekT			87 / 1e-21
O142-O142_10			84 / 1e-20

ncbi CAZy

- Reference [1]

NMR
1H-NMR		5.13 4.16 3.89 3.33 3.68 1.25 5.22 4.27 4.00 3.61 3.79 1.34 4.86 3.85 3.79 3.55 4.02 1.24 4.77 3.83 3.63 3.48 3.48 3.73 3.95 2.07 5.09 3.60 3.79 3.45 3.93 3.77 3.84
Solvent:	D2O
Temp:	40C
pD:
Ref:	TSP 0 ppm
Comment:	1H-NMR [2] Chemical shifts are given for the deacetylated structure
13C-NMR		102.20 79.60 71.10 73.60 70.50 17.70 101.80 75.60 74.80 71.60 70.30 18.00 102.40 71.80 78.30 72.90 70.20 17.50 103.10 56.90 82.70 69.70 77.20 62.20 23.50 175.40 95.70 72.30 74.20 70.80 72.90 61.80
Solvent:	D2O
Temp:	40C
pD:
Ref:	Acetone 31.45 ppm
Comment:	13C-NMR [2] Chemical shifts are given for the deactylated structure

Non-Sugar Components
- Acetate

Enzymes
Flippase		MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAIALVGYAGIFDVGLTRSVIREIAIHRD NHHERTKVISTSTSFLVLFSCFGAFLLLIFSDGIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSA ILEGDEKFGIVNIQKSISSSCIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFC YKTFKRLFFFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLS NIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQISSFNILNVLLIGFFFNALAMIP FSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVILDALLLQWLYTRMCSVYEN
Trembl:	B5L494
Polymerase		MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFILLIFNLVTFNFSITQKCVSLFIWLLLF PFYGFFVGLLAGNKINDILFDVQPYLFMLSLIYLFTLRYTLKLFLCEIFIKIVNAFALYGSLLYISYIIL LNFGLLNFNLIYEHLSLTSEFFFRPDGAFFSKSSYFFGAGAIISFVNKKYLKWLLIVLAILLTESRGVLL FTTLSLLLASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLFGRGF GSFILDRLRIGIVPLEILQKTGVIGVYGSLVPMLLIFVKGYFLNSTKTSLMMSLILFFSITVSITNPFLF TPMGIFIIGVVVLWVFSIENIQISNNLTSGAK
Trembl:	B5L497

Literature
First Author: Liu, B. "Structure and genetics of Shigella O antigens". FEMS Microbiology. 32. (2008), pp 627-653 PubMed: [18422615]
First Author: Perepelov AV. "Structures of the O-antigens of Escherichia coli O13, O129, and O135 related to the O-antigens of Shigella flexneri". Carbohydrate Research. 345(11. (2010), pp 1594-9 PubMed: [20546712]