O-Antigen Description

Serogroup:	O168
Serotype H:
Serotype K:
Strain:
Structure:	aLFuc(1->3) \| ->4)aDGlcNAc(1->4)aDGlcA(1->3)aLFuc(1->3)bDGlcNAc(1->
Comment:	- Structure [1] - aLFuc is O-acetylated in position 2/3/4, in total 50% - aDGlcNAc is O-acetylated in position 6, in total 50%

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfeV

->4)aDGlc

GT Name	Donor	Acceptor	Score / e-value
O41-O041_06			121 / 2e-34
O11-WdaG			114 / 1e-31
O125-O125_13			114 / 2e-31
O36-O036_15			112 / 6e-31
O84-O084_13			111 / 1e-30
O41-O041_12			110 / 2e-30
O43-O043_13			108 / 2e-29
O159-WfgS			106 / 6e-29
O11-WdaD			105 / 1e-28
O168-WfeY			103 / 6e-28
O156-O156_14			103 / 6e-28
O184-O184_15			102 / 3e-27
O173-WcmU	aLFuc(->	->4)aDGlc	98 / 1e-25
O43-O043_04			92 / 8e-24
O157-WbdO			86 / 1e-21
O36-O036_08			84 / 9e-21

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfeW

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfeX

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfeY

->4)aDGlc

GT Name	Donor	Acceptor	Score / e-value
O156-O156_14			508 / 0.0
O84-O084_13			484 / 0.0
O159-WfgS			483 / 0.0
O41-O041_12			474 / 0.0
O36-O036_15			467 / 0.0
O125-O125_13			263 / 4e-89
O184-O184_15			263 / 3e-89
O11-WdaG			262 / 5e-89
O43-O043_13			258 / 3e-87
O11-WdaD			125 / 4e-36
O43-O043_04			112 / 3e-31
O173-WcmU	aLFuc(->	->4)aDGlc	108 / 1e-29
O168-WfeV			103 / 6e-28
O111-WbdL	aDGlc(1->	->4)aDGal	100 / 2e-26
O36-O036_08			95 / 9e-25
O41-O041_06			83 / 1e-20

ncbi CAZy

- Reference [1]

NMR
1H-NMR		5.11 3.68 3.95 3.79 4.72 1.25 5.17 4.13 3.91 3.87 3.72 3.71 3.83 5.17 3.57 3.73 3.73 4.18 4.97 3.88 3.89 3.91 4.31 1.14 4.55 3.80 3.63 3.31 3.44 3.59 3.96
Solvent:	D2O
Temp:	30C
pD:
Ref:	TSP 0.0 ppm
Comment:	1H-NMR [1]
13C-NMR		99.70 69.20 70.70 73.30 68.00 16.90 99.60 55.30 73.30 79.30 73.30 60.80 102.00 73.30 74.60 79.30 73.70 175.70 101.60 68.70 74.60 73.70 68.40 16.50 101.50 57.00 81.90 77.60 63.20
Solvent:	D2O
Temp:	30C
pD:
Ref:	Acetone 31.45 ppm
Comment:	13C-NMR [1]

Enzymes
Flippase		MFRQINFKNLEIMKQILISFSFRLMGPVIMLLTTPQLLSTLGNVEYGVWSLLITTISWLTICDFGISNQI RNESKILIVQDNKESIREIYTAAVIISFFITLLCCVIISFINIDLIYKLFLSCVFLFAIETNICRALCLS LNKPELASAVTNIPNLFLLIYVFCYSSFNINNVMDIYCYGSAMMFVSIFIILYKFNIKLFWPVFHPSYLR FSIMKYSLLRGGGGFFMVQLVSMGLIISDRYIISSIFGVEDVTKYDLIFKVSNFCIMFFSIVNMVLWSKF TEFWMQGFYTKIKVIFKVFDLCFLLLVIVLILILNHIHWIFELWLNVTNENYNEVYITGIVLYLVGYYWM SAYSSFLNGISIIKKQFIIQGGGLLIKAITLLIAMNILKVFKVEYIILLGGACLFIVGFLFRKCAHDKIN SCI
Trembl:	B5L3Q8
Polymerase		MNCKFNIWDIQLGLILISVLSYAFLNLGFLPLNISHLIILFVLITLSVNLLISQSIKITGLFLTLFVSFI LLSAYSLLKYYDVQKVKNIINFFIFFSVAAIVINNCSADKIIKFYYKLTKIFIFFALLQWVLYYLNIGTL YTYSFLGLKEVNISTSGYLIRLFSIASEPAALCGILLPAIYLSINRIVNKGKEVTLSYSVIVLFVILNTF SLVGYIYIIICLIVALYVGNKISLSKIFIFTICLALLVFILFQSDSIQQRLNEITSLDKMASSDNLSVIA IYSNLQIALISLSDNLIFGGGIFSHPYTYDHYISQLYAGGGPRMELNKDDAASLYIRALSETGILGFCIL NGLIIYLMKRCDKSKINYPYNIAFTIAFALLGIRAGSVNYIIIWFYFFSAIRFVNEGRLK
Trembl:	B5L3R2

Literature
First Author: Liu, B. "Structure and genetics of Shigella O antigens". FEMS Microbiology. 32. (2008), pp 627-653 PubMed: [18422615]
First Author: Perepelov, AV. "Antigenic polysaccharides of bacteria: 41. Structures of the O-specific polysaccharides of Shigella dysenteriae types 4 and 5 revised by NMR spectroscopy].[Article in Russian]". Bioorg. Khim. 34(4). (2008), pp 513-21 PubMed: [18695724]
First Author: Perepelov, AV. "Close relation of the O-polysaccharide structure of Escherichia coli O168 and revised structure of the O-polysaccharide of Shigella dysenteriae type 4.". Carbohydrate Research. 342(17. (2007), pp 2676-81 PubMed: [17880932]
Bioorg. Khim. (1977), pp 1226–1233