O-Antigen Description

Serogroup:	O29
Serotype H:	10
Serotype K:
Strain:
Structure:	aDGlc(1->6)aDGal(1->3) \| ->3)DGro(1-P-6)bDGlc(1->4)aLFucNAc(1->3)bDGlcNAc(1->
Comment:	- Structure [2]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WffX

ncbi CAZy

- Reference [1]
- Glycerophosphotransferase

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WffY

aDGlc(1->

GT Name	Donor	Acceptor	Score / e-value
O56-WfaQ			188 / 8e-58
O173-WcmX	aDGlc(1->	->2)bDGlc	177 / 1e-53
O152-WfgE			173 / 4e-52
O24-WfaO	aDGlc(1->	->2)bDGlc	166 / 3e-49
O144-O144_03			162 / 5e-48
O81-O081_10			153 / 8e-45
O169-O169_04			140 / 9e-40
O183-wbaT			139 / 2e-39
O85-WfbQ			132 / 7e-37
O130-WffI			127 / 4e-35
O66-WfaB			125 / 2e-34
O18-wcxD			125 / 2e-34
O102-WelN	aDGal(1->	->6)bDGlc	115 / 8e-31
O27-WejX			113 / 3e-30
O187-O187_10			103 / 4e-27
O34-O034_11			100 / 7e-26
O65-O065_10			89 / 9e-22
O110-O110_03			86 / 9e-21

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WffZ

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfgA

GT Name	Donor	Acceptor	Score / e-value
O108-O108_14			392 / 2e-137

ncbi CAZy

- Reference [1]
- Similarities from PSI-Blast

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbuB

aLFucNAc(1->

->3)bDGlcNAc

GT Name	Donor	Acceptor	Score / e-value
O15-WbuB			632 / 0.0
O145-WbuB	aLFucNAm(1->	->3)bDGlcNAc	629 / 0.0
O108-O108_18			605 / 0.0
O151-WbfG			598 / 0.0
O118-WbfG			598 / 0.0
O25-WbuB			556 / 0.0
O4-WbuB	aLFucNAc(1->	->3)bDGlcNAc	556 / 0.0
O177-WbuB			555 / 0.0
O172-WbuB	aLFucNAc(1->	->3)aDGlcNAc	544 / 0.0
O12-O012_09			514 / 0.0
O26-WbuB	aLFucNAc(1->	->3)bDGlcNAc	503 / 0.0
O98-WbwW	aLQuiNAc(1->	->3)bDGlcNAc	446 / 3e-158
O181-O181_07			378 / 6e-132
O123-WbwH			366 / 7e-127
O186-O186_16			329 / 1e-112
O163-O163_11			326 / 2e-111

ncbi CAZy

- Reference [1]

NMR
1H-NMR		4.93 3.58 3.57 3.45 3.76 3.76 3.86 5.09 3.69 3.89 4.09 4.32 3.65 3.89 4.64 3.42 3.52 3.51 3.49 4.02 4.09 4.98 4.45 4.06 4.22 4.50 1.31 4.49 3.87 3.69 3.65 3.47 3.76 3.94 3.85 3.90 3.98 3.64 3.97
Solvent:	D2O
Temp:	30C
pD:
Ref:	Acetone 2.225 ppm
Comment:	1H-NMR [2]
13C-NMR		98.50 72.50 75.20 71.00 73.00 62.10 100.80 69.60 70.80 71.20 71.00 67.20 104.50 75.20 76.70 70.10 76.40 65.70 99.50 50.40 72.10 79.20 69.40 16.80 103.10 56.90 79.90 70.10 77.30 62.30 67.70 70.80 72.30
Solvent:	D2O
Temp:	30C
pD:
Ref:	Acetone 31.45 ppm
Comment:	13C-NMR [2]

Enzymes
Flippase		MSNKNKTFKDFINYFLGDLFVKGFMFISLPLLSRIMSPEDYGRMSLINAAVMILYVFISLNLQNAVINAY MKNEVDFPVYLGSVLWGLTAAQVLLVALSIYFAVPLGMLLSISKYDVYWVVAICILLSYIYIYTSYLQGA RLSSSFVKLNIFSKISEVVVIFVFAWFLTQDKYLAKVYAQLVISFILLTYVLKKLKKIAVFKFNVRYFIS ALAFSSPLIIHVLSNALLSQVDRLFIAKMLGEGQAGIYSFAYNIGMCILVVVMAWNSSWQPKLYKLIDSK DNGKIIRIVDVSSLLLLIVSFLSILFSKQMVEVLADNRYRESISVVPVILIGNSLIHIYLSYVNFTFYKK KTIYVSIGTLLAVAINIALNYILIPIYGIHGAAWATVIAYFMLAFFHYLIATIMLKANPLSLFLLLWYSA LLLASYFLVIYLDSLSLWISLSIKAMIIFIILIILMKTKIYNELKE
Trembl:	B5L3I1
Polymerase		MTVSIYLLLLAFVFLLAMSDFFIIADARNRFLLYIILFSLLVTFIGLRYQTGLDWLFYNNLFNGEGFSLA IEPGYYFFSYVSSFLMGYWIYQALITAVLIICLKTFFEKNTKNYLFCIGFFFLYQFIFVTEAIRQIIALS IILVAYKKFYDGKKLQFHMLTILACSFHISAVIVFILIPFLKRRNIYILKILTIVGLVLAIFSVYPVDYL IQLLSLLPAGGYIEKIRWYSQDDYAGSVLTFSLVFKVFVVLLFDYRFKSIKSHGQSLINARAYDFIYTSV YLMIFMDVYLGRFGTISTRLDVYFIPCFLIALNHLINEHKQGVSRFIFFFVVMVYFTINYLSIMNGYYFE KFYSPYQNYITEFLNPGSYSDRGWDVRYYFSNKELLQ
Trembl:	B5L3I2

Literature
First Author: Liu, B. "Structure and genetics of Shigella O antigens". FEMS Microbiology. 32. (2008), pp 627-653 PubMed: [18422615]
First Author: Perepelov, AV. "Structure of a teichoic acid-like O-polysaccharide of Escherichia coli O29". Carbohydrate Research. 341(12. (2006), pp 2176-80 PubMed: [16784735]