O-Antigen Description

Serogroup:O124
Serotype H:
Serotype K:72
Strain:
Structure:
bDGlc4RLac(1->6)aDGlc(1->4)
                         |
                  ->3)bDGal(1->6)bDGalf(1->3)bDGalNAc(1->
Comment:- The correct structure is given in the structural reinvestigation [4]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfeQ

bDGalNAc(1->

GT NameDonorAcceptorScore / e-value
O164-WfeQ


674 / 0.0
O12-O012_O2


118 / 4e-32
O171-O171_11


90 / 2e-22
O165-WcnW


89 / 3e-22
O124-WfeS


88 / 2e-21
O164-WfeS


88 / 2e-21
O128-WbsK

bDGalNAc(1->

->4)aDGal

87 / 1e-21
O46-O046_08


86 / 3e-21
O134-O134_08


86 / 4e-21
O173-WcmW

bDGlc(1->

->3)bDGlcNAc

85 / 7e-21
O112-WfeM


81 / 2e-19
O27-WejU


81 / 1e-19
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfeR

GT NameDonorAcceptorScore / e-value
O164-WfeR


540 / 0.0
O23-O023_05


111 / 6e-31
O82-O082_07


106 / 6e-29
O38-O038_07


92 / 7e-24
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfeS

GT NameDonorAcceptorScore / e-value
O164-WfeS


692 / 0.0
O171-O171_11


128 / 1e-35
O160-O160_04


113 / 3e-30
O112-WfeM


102 / 2e-26
O185-O185_09


99 / 3e-25
O28-O028ab_05


90 / 2e-22
O143-WfdV


87 / 2e-21
O117-WbeA


84 / 2e-20
O12-O012_O2


83 / 7e-20
O107-WclW


82 / 1e-19
O112-WfeF


81 / 8e-19
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfeT

bDGalf(1->

->3)bDGalNAc

4 | 1
GT NameDonorAcceptorScore / e-value
O164-WfeT


622 / 0.0
O85-WfbU

bDGalf(1->

->3)bDGlcNAc

325 / 8e-112
O167-WfdK

bDGalf(1->

->3)bDGlcNAc

307 / 7e-105
O96-O096_08


305 / 7e-104
O170-O170_08


287 / 5e-97
O42-WbeY

bDGalf(1->

->3)aDGlcNAc

219 / 2e-70
O28-WbeY

bDGalf(1->

->3)aDGlcNAc

218 / 4e-70
O170-O170_O4


165 / 8e-50
O167-WfdJ


103 / 6e-27
O96-O096_04


93 / 1e-23
ncbi CAZy
- Reference [1]
NMR
1H-NMR5.09 4.10 4.03 4.05 4.01 3.74 4.08 4.92 3.51 3.73 3.65 4.32 4.05 4.31 4.71 3.94 3.81 4.05 3.67 3.79 3.86 2.04 4.50 3.67 3.83 4.25 3.77 3.82 3.87 4.47 3.35 3.62 3.39 3.48 3.84 3.91 4.34 1.36
Solvent: D2O
Temp: 45C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [4]
13C-NMR110.00 82.10 78.00 84.20 70.90 72.10 100.00 72.80 73.50 69.70 71.30 68.80 104.20 52.30 79.30 68.80 75.70 62.00 23.20 175.40 104.30 71.70 81.50 76.20 76.20 60.80 103.50 74.00 76.70 77.70 75.70 61.90 78.70 19.70 182.20
Solvent: D2O
Temp: 45C
pD:
Ref: Acetone 67.4 ppm
Comment: 13C-NMR [4]
Enzymes
FlippaseMSRNKIIIKNTMFLATRTLVSLSVSFYTTRIVIQQLGAADYGLFNIIYGVVTFFTFVVTAMNDSVQRYIA IGVGSQKISVIRDAVKNSMFIFVISAFILAVCLLLARGVIIHNILNIPKDSIENASVLYLVAVFSITILI IQTPLNAMVLAYEKMSFYAYMMIFEMVAKMSMALLLTLLEKDKVIVYSILLGSISFFNLLVYLCYCLICF KKSMFGGRIKFRVLKEISTFSFWNIFGNFSYMCRVQGVNIVINMFYAIAVNAAYAISITVLNAINTLTQS LITALRPQIFKSYGECDLKRYNHLVLFGSKYTFSILFLLSSPVILCADELLKIWLDIVPDYTVEFVRLVI VVAFIDSFSYSMIAGIQATGRIKTYQLVVSLIVLINLPLTFILFKAGNNVLSMFYPFIVTAIINQGLRLY FIYINAGFDYKKYFTVVIYPCLLAVCLSLVTDISIKKMLPFNSVIDVLIVCIFIFSFNTIIFYWVVVS
Trembl: B5L449
PolymeraseMYFLLFFSIFYFVYVILIGTLCHNYKALLIFSLIPLTIVSGIRYNVGFDFMSYVDYFNQLKYSDDIYLDS TFKYISYFTYYIGGNEQILFLIYAVFYSVALYFLIKLVLENYITEINNFYTIGLVLSFYSFYFLLSFNQI RAVLSALILCYGLLKNKIDFSFIITITLSILFHSAAMFILPLYFVLRKINVTVLLVIFPFLVVASFFNIF SDIVRFILTLLNSRFLTYFNSEYFVPRTGMEKMYSMISMIIVLGMVVCLSKLLPKKFDLMIKFVILFVLL RAMSIDILIFARFSDFLKPMAIILIFTAVYFSSNKVRPRIILPFYLIMTLFLCIFNIIIGSNITKGNYYT YGYNICFFGNKCIDKFY
Trembl: B5L454
Literature
  • First Author: Liu, B. "Structure and genetics of Shigella O antigens". FEMS Microbiology. 32. (2008), pp 627-653 PubMed: [18422615]
  • First Author: Dmitriev, BA. "Cell-wall lipopolysaccharide of the "Shigella-like" Escherichia coli 0124. Structure of the polysaccharide chain". European Journal of Biochemistry. 64(2). (1976), pp 491-498 PubMed: [819266]
  • First Author: Dmitriev, BA. "Complete structure of the repeating unit of the O-specific polysaccharide chain of Shigella dysenteriae type 3 lipopolysaccharide". Carbohydrate Research. 56(1). (1977), pp 207-209 PubMed: [328145]
  • First Author: KHM Jonsson. "Structural studies of the O-antigenic polysaccharides from Shigella dysenteriae type 3 and Escherichia coli O124, a reinvestigation". Carbohydrate Research. 341. (2006), pp 2986-2989 PubMed: [17081507]