O-Antigen Description

Serogroup:	O179
Serotype H:
Serotype K:
Strain:	E4378
Structure:	->4)aDGlcA(1->3)bDMan(1->4)bDMan(1->3)bDGlcNAc(1-> \| bDGlc(1->2)
Comment:	- Strain is given for the gene sequence study in reference [2] - Structure presented at the 29th International Carbohydrate Symposium, Lisbon, Poster no BS2. 2018.

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O179_03

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O179_06

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O179_07

bDMan(1->

->4)bDMan

GT Name	Donor	Acceptor	Score / e-value
O6-Orf5	bDMan(1->	->4)bDMan	162 / 2e-48

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O179_08

bDMan(1->

GT Name	Donor	Acceptor	Score / e-value
O106-WbaD			381 / 4e-133
O73-WbaD	bDMan(1->	->3)aDGlcNAc	381 / 4e-133
O77-WbaD	bDMan(1->	->3)aDGlcNAc	381 / 4e-133
O44-O044_03			369 / 1e-128
O17-O017_03			369 / 1e-128
O6-Orf7			350 / 2e-121
O62-O062_12			330 / 2e-113
O59-WclC			216 / 6e-69
O176-O176_05			87 / 1e-21
O119-WekK			87 / 2e-21

ncbi CAZy

- Reference [2]

NMR
1H-NMR		5.42 3.63 3.89 3.72 4.09 4.83 4.44 3.90 3.86 3.48 3.79 3.94 4.71 3.92 3.81 3.81 3.54 3.76 4.64 3.35 3.52 3.52 3.36 3.80 3.93 4.63 3.86 3.74 3.59 3.52 3.80 3.94
Solvent:	D2O
Temp:	48C
pD:
Ref:	TSP 0.0 ppm
Comment:
13C-NMR		99.8 72.1 71.3 81.6 73.2 175.5 101.2 78.6 76.2 67.6 76.8 61.5 101.3 70.9 72.1 77.5 75.7 61.5 104.4 74.3 76.1 69.7 76.5 60.7 100.90 55.00 84.6 69.0 76.1 60.7
Solvent:	D2O
Temp:	48C
pD:
Ref:	Dioxane 67.4 ppm
Comment:

Enzymes
Flippase		MNFLVYLALSIVSKLIASVREFGVGFIWGTGSIVDAFILAMMLPMFFIDIASVMFNSTYIGIYVNSANKE RRFFFKDVFYFTLIYVLFTNIIVIFFLKEYIAYSDIPIETKHIIDNIIIYIVVYYSLLAFCEFYKAHLMA CEKQKMMPLPMICANIVFISTMYYFSEGANDIALVSCFVFSSVIQFILYIPLIIKKDSVYIMNSSVSHSR EANKKLFLKNLVPVFFNAAVNQSSKITDKVIASSLIIGSLSSLYFAQQLYALYVNIIIVSVLSFAYPKIC KKANENKYEFSSYIVQVIIVLFVLLVYPVMLGWRYAQDIFKFILVNSTTQNIKVVSEMFMVLLSVVVFES ISASLKRAFWALNKMKVTVYISGFTVIINIILSVLLSSYYGPKGLVYSYCAATILSTMLLIFYFNKVSKI TIPKNIFLPIMTASIISVTFYILLGFFRIDLNKPISLLIPGLIVVLGMFLLFVCFNKNSISKVLKE
Trembl:	A0A0A8J7J6
Polymerase		MFTISPVFFENITFQGSRTLADVLFCISLLLIICKHKKVNISLLGIVYIIIVLVIMSVNA ALGFSNASTTINNELFDKLTWLILDRNINEFIPFGDYYRNILIYTRYLLIPFYFWMGLYY SKLVGKERAIVTLYNGLIICLLLNMLYGIYNDDDRLTGFFENTAALSSLALLCMLLSLFT KSKYKIILSWVIGIIALLMSQTASAFIGLTAVVLILVFNIRRHHKKTVYITSLLSVVIAS IGLMPEIMEFIARYMYTGSLINRINTWSTIINYFDSYQVAILGLGSFPLFADNIFIWLIS GFGIFSFVIYMYLVSIGNKSQKSALFISLLLWQGVLFPGFIMPYMLMTTFITLGILSNNK GCENECI
Trembl:	A0A0A8J9D5

Literature
First Author: Scheutz F. "Designation of O174 and O175 to temporary O groups OX3 and OX7, and six new E. coli O groups that include Verocytotoxin-producing E. coli (VTEC): O176, O177, O178, O179, O180 and O181". Acta Pathologica, Microbiologica, et Immunologica Scandinavica. 112. (2004), pp 569-584 PubMed: [15601305]
First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
First Author: Fontana C. "Structural Studies and Biosynthetic Aspects of the O-Antigen Polysaccharide from Shiga-toxin-Producing E. coli O179". Carbohydrate Research. inprep. (2018), pp Poster