O-Antigen Description

Serogroup:O121
Serotype H:
Serotype K:
Strain:
Structure:
->3)bDQui4NGlyAc(1->4)aDGalNAcA6N(1->4)aDGalNAcA(1->3)aDGlcNAc(1->
Comment:- Structure [1]
- Qui4NGlyAc=4-acetylglycylamido-4,6-dideoxy-glucose
- 60% acetate at O-3 in aDGalNAcAN
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbqE

GT NameDonorAcceptorScore / e-value
O65-O065_08


106 / 4e-28
O116-O116_07


105 / 7e-28
O123-WfbE


98 / 3e-25
O186-O186_10


97 / 4e-25
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbqH

->4)aDGal

GT NameDonorAcceptorScore / e-value
O116-O116_10


122 / 1e-33
O3-WclR


112 / 5e-30
O142-O142_12


106 / 5e-28
O113-WbnE


104 / 2e-27
O111-WbdM

aCol(1->

->3)aDGlc ->6)aDGlc

101 / 2e-26
O87-WfgO


100 / 8e-26
O103-WbtF


96 / 2e-24
O69-O069_12


96 / 2e-24
O18-wekW


91 / 2e-22
O40-WffO


89 / 6e-22
O101-O101_06


88 / 1e-21
O18-wekV

aDGlc(1->

->4)aDGal

86 / 7e-21
O89-O089_06


85 / 2e-20
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbqI

aDGalNAcA(1->

->3)aDGlcNAc

GT NameDonorAcceptorScore / e-value
O138-WfaH

aDGalNAcA(1->

->3)bDGlcNAc

304 / 4e-103
O101-O101_07


187 / 5e-58
O89-O089_07


182 / 8e-56
O116-O116_11


171 / 1e-51
O100-O100_11


168 / 9e-51
O103-WbtG


167 / 3e-50
O133-O133_08

aDGalA(1->

->3)aDGlcNAc

166 / 9e-50
O158-wcmT


162 / 2e-48
O10-O010_12


150 / 7e-44
O111-WbdH

aDGal(1->

->3)bDGlcNAc

137 / 3e-39
O52-Orf16


125 / 7e-35
ncbi CAZy
- Reference [2]
NMR
1H-NMR5.37 4.22 4.01 4.46 4.41 5.09 4.29 4.13 4.50 4.83 5.04 3.97 3.86 3.70 4.15 3.82 3.80 4.54 3.45 3.78 3.78 3.45 1.10 3.76
Solvent: D2O
Temp: 45C
pD:
Ref: Acetone 2.225 ppm
Comment: 1H-NMR [1], de-O-acetylated
13C-NMR99.47 50.21 66.87 78.37 71.03 172.39 99.54 50.75 68.56 79.35 71.73 173.93 97.30 52.84 79.74 71.79 72.22 60.64 104.56 73.76 77.71 57.33 71.73 17.25 43.73
Solvent: D2O
Temp: 45C
pD:
Ref: Acetone 31.07 ppm
Comment: 13C-NMR [1], de-O-acetylated
Non-Sugar Components
- Acetate - Glycine
Enzymes
FlippaseMGAESYGLIGFFTMLQALFGLLDLGLTPTIGRETARYHGGTMTVLDYRKLLRTLHILFFT IATIGGVFLFFLAPIISVHWLKVTTISIDTVNYCVKIMAFSIALRWIGGLYRGIISGSER LDWLSYLNIFITTLRFIIIFPVMKVKGYTPFVFFTYQLIVAIIEYLSLLCLSTLLTPKLK SANEKIGFSFSPIMSLMRFSLTVAFTSSIWILITQSDKLILSGILELSEYGHFTLSVLLA SGILMINIPVTNSILPRLARLHAENKHDELLRIYKNTTMLVCILGVSASLVVAIYAEPLL LIWTGDAEVSASASPILSLYAIGNGLLAVSAFPYYLQYAQGRLKYHFWGNIVMFFMLIPT IIVLARNFGGVGAGYAWLIVNVFYLFVWTALVHHKLIPGLHLSWLMSIGMITIPTGIIVF FLSFIVRFSGNRLHDIIVLGLISILALMVSIVSCWLIKRMNLGV
Trembl: Q84DG4
PolymeraseMINLNPVAILTLLYLSINFLSMLIGCSSGEIQVETSIFRVSEESLIYSFLLQAICLIFLY YIYKYFTNRISYPPLTFKAKWGRALLIIQIAFIIFNTQMGVNTAGSVERIEGQSLSNYLF IILQPDILVAVISVCLNSGFLFWTNILVYLLSMFLRGWMGGTFVILFLILSRYQNLRISL KTFLVSLCSLLLLFSILPALIEAKWAMRTGISLSVFISNMSSYVTPENYYAGINYLLNRF QHVGHLALIYENADDLFKKYNAGYFSSYYMDGIPQYLLVKMYNLDMYKLSFYLVQYFFDI TEPTWNINTGVVGWLYILRYESILFAFYIMLLLLVPYYVVSRFAGKRMLSVLACFSIIYL FHGWLGAYVNLAFYACIISLLANIRLYRTVYIPCEK
Trembl: Q84DG1
Literature
  • First Author: Parolis, H. "Structural studies on the Shigella-like Escherichia coli O121 O-specific polysaccharide". Carbohydrate Research. 303(3). (1997), pp 319-25 PubMed: [9373937]
  • First Author: Fratamico, PM. "Sequence of the Escherichia coli O121 O-antigen gene cluster and detection of enterohemorrhagic E. coli O121 by PCR amplification of the wzx and wzy genes". Journal of Clinical Microbiology. 41(7). (2003), pp 3379-3383 PubMed: [12843098]
  • Methods Microbiology. 14. (1984), pp 43-112