O-Antigen Description

Serogroup:	O149
Serotype H:
Serotype K:
Strain:
Structure:	->3)bDGlcNAc4,6(S)Pyr(1->3)bLRha(1->4)bDGlcNAc(1->
Comment:	- Structure [2] - 4,6(S)Pyr=(S)-4,6-pyruvic acid acetal

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbuU

GT Name	Donor	Acceptor	Score / e-value
O55-WbgP			92 / 1e-23
O55-WbgP			92 / 8e-24
O169-O169_03			84 / 2e-20
O183-wbaS			82 / 4e-20

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbuV

bLRha(1->

->4)bDGlcNAc

2 | 1

GT Name	Donor	Acceptor	Score / e-value
O180-wfcU	bLRha(1->	->4)aDGlcNAc	392 / 4e-137
O2-wegR			363 / 1e-125
O50-O050_13			359 / 2e-124

ncbi CAZy

- Reference [1]

NMR
1H-NMR		4.82 4.82 4.75 2.02 2.02 1.30 1.30 1.50
Solvent:	D2O
Temp:	85C
pD:
Ref:	TSP 0 ppm
Comment:	1H-NMR [2] partial data
13C-NMR		103.20 102.20 101.30 56.80 55.60 23.30 23.20 17.50 101.30 25.40
Solvent:	D2O
Temp:	70C
pD:
Ref:	Dioxane 67.4 ppm
Comment:	13C-NMR [2] partial data

Non-Sugar Components
- Pyruvic acid

Enzymes
Flippase		MNSNKRQTLFFSLDQIIDSGLVFIFIAIGGNLLDKSDMANVVLFQSVALVSVLFCSCFTTQYLLLRYKKQ SSLFWLKLFLFFCIITMLIVSLWFQSAEILFLFFVGISSEYIKRYCFYINNSCLSFFSTIITAFIFFVFI LMCWLNFMRINSTIYIYIYSGAKLIPLTIICCFLFIKTKFNSDGEYEMPSLWYAITDSLRFGGVFSVITI IYWITNQGFFIFFHDRMPAAELVEIRVTQNVFGVVTMLIALYDSMFLKKNIENKNKIFDWRSYFKFLVIS IVLITFNFILLYILSITIYKHLDVFKYSICLALAQFFYLSARMPVLILKLRYSLSIILWLYVCSLFLSSC YLFLKHNDYDFQYIVQSITLSNFFIFLFSLLIVFYKERIYGKNY
Trembl:	Q45XG7
Polymerase		MLVTILALFLFIQLLYLISYKRNFNTKSINDILSFIIFLFLIVLFFFRNEDFGADTQNYLNEFDEYCSNP SGYIGVDYTYKYIFDLINFLMLDSCHISWLIWIWPIFVIGCIFSAITIFRVDKIYIIALFSSFIGIELLT NALRQGFSIAVLLLSFCLFIKRRYFLFLIAVTISFLFHQASILIVSIFIISRCSIRIVVPSIVVGVFLLF ATNILDVVPWVIEFKKLIYKYLPYADEDFIIRIISIINTLCLCVLYLIVMIKSHCKDKIINNIVINATFV CCIASIVPYLGFRIIYGVYPLLLLLTYCSIRNYIGRVSSYNYLSLSICINIFISIMWLCGSSHMNKIPFV SIII
Trembl:	Q45XG5

Literature
First Author: Liu, B. "Structure and genetics of Shigella O antigens". FEMS Microbiology. 32. (2008), pp 627-653 PubMed: [18422615]
First Author: Adeyeye, A. "Structural studies of the Escherichia coli O-149 O-antigen polysaccharide". Carbohydrate Research. 176(2). (1988), pp 231-236 PubMed: [3046745]
First Author: Han, W. "DNA microarray-based identification of serogroups and virulence gene patterns of Escherichia coli isolates associated with porcine postweaning diarrhea and edema disease". Applied and Environmental Microbiology. 73(12). (2007), pp 4082-4088 PubMed: [17449692]