O-Antigen Description

Serogroup:O157
Serotype H:7
Serotype K:
Strain:C664-1992
Structure:
->2)aDRha4NAc(1->3)aLFuc(1->4)bDGlc(1->3)aDGalNAc(1->
Comment:- Structure from [2]. [1] gave wrong structure which was subsequently revised.
- Salmonella urbana and S. godesberg have the same backbone substituted with glucose on position 4 of the GalNAc residue [5].
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbdN

bDGlc(1->

GT NameDonorAcceptorScore / e-value
O24-WfaM

bDGlc(1->

->3)bDGalNAc

431 / 5e-155
O130-WffJ


241 / 3e-80
O21-WclQ


234 / 2e-77
O102-WelO

bDGlc(1->

->3)bDGalNAc

226 / 2e-74
O134-O134_08


225 / 2e-74
O27-WejU


225 / 3e-74
O61-WeiL


224 / 4e-74
O46-O046_08


223 / 2e-73
O81-O081_11


218 / 4e-71
O140-O140_14


184 / 2e-58
O143-WfdV


178 / 7e-56
O112-WfeI


176 / 2e-55
O38-O038_09


166 / 3e-51
O30-O030_06


137 / 3e-40
O152-WfgD

bDGlc(1->

->3)bDGlcNAc

135 / 1e-39
O93-O093_02


131 / 7e-38
O161-weiG

bDGlcA(1->

->3)bDGlcNAc

129 / 2e-37
O182-O182_10


129 / 3e-37
O91-WbsA


125 / 7e-36
O165-WcnW


124 / 2e-35
O82-O082_09


113 / 2e-31
O79-WbdX


112 / 6e-31
O173-WcmW

bDGlc(1->

->3)bDGlcNAc

111 / 1e-30
O128-WbsK

bDGalNAc(1->

->4)aDGal

105 / 5e-28
O55-WbgP


102 / 2e-27
O55-WbgP


102 / 2e-27
O56-WfaP

bDGlc(1->

->3)bDGlcNAc

93 / 7e-24
O130-WffH'


88 / 3e-22
O125-O125_04


82 / 7e-20
O34-O034_10


82 / 6e-20
O183-wbaS


80 / 2e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbdO

GT NameDonorAcceptorScore / e-value
O173-WcmU

aLFuc(->

->4)aDGlc

90 / 5e-23
O43-O043_13


88 / 2e-22
O43-O043_04


87 / 5e-22
O168-WfeV


86 / 1e-21
O11-WdaG


86 / 2e-21
O125-O125_13


85 / 2e-21
O184-O184_15


84 / 5e-21
O41-O041_06


83 / 1e-20
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbdP
NMR
1H-NMR4.42 3.16 3.42 3.67 3.38 3.75 4.80 3.73 3.69 3.94 3.58 1.03 4.95 4.18 3.92 4.12 4.07 1.00 5.09 3.79 3.90 3.87 0.00 3.59
Solvent: D2O
Temp: 70C
pD:
Ref: Acetone 2.225 ppm
Comment: 1H-NMR [1]
13C-NMR103.40 73.00 73.90 77.80 74.70 59.50 98.90 66.70 71.40 76.60 0.00 16.40 99.50 66.60 70.80 48.50 68.20 14.70 100.40 52.80 67.80 77.70 0.00 60.90
Solvent: D2O
Temp: 70C
pD:
Ref: Acetone 31.07 ppm
Comment: 13C-NMR [1]
Enzymes
FlippaseMIMNKIKKILKFCTLKKYDTSSALGREQERYRIISLSVISSLISKILSLLSLILTVSLTL PYLGQERFGVWMTITSLGAALTFLDLGIGNALTNRIAHSFACGKNLKMSRQISGGLTLLA GLSFVITAICYITSGMIDWQLVIKGINENVYAELQHSIKVFVIIFGLGIYSNGVQKVYMG IQKAYISNIVNAIFILLSIITLVISSKLHAGLPVLIVSTLGIQYISGIYLTINLIIKRLI KFTKVNIHAKREAPYLILNGFFFFILQLGTLATWSGDNFIISITLGVTYVAVFSITQRLF QISTVPLTIYNIPLWAAYADAHARNDTQFIKKTLRTSLKIVGISSFLLAFILVVFGSEVV NIWTEGKIQVPRTFIIAYALWSVIDAFSNTFASFLNGLNIVKQQMLAVVTLILIAIPAKY IIVSHFGLTVMLYCFIFIYIVNYFIWYKCSFKKHIDRQLNIRG
Trembl: O85337
PolymeraseMKSAAKLIFLFLFTLYSLQLYGVIIDDRITNFDTKVLTSIIIIFQIFFVLLFYLTIINER KQQKKFIVNWELKLILVFLFVTIEIAAVVLFLKEGIPIFDDDPGGAKLRIAEGNGLYIRY IKYFGNIVVFALIILYDEHKFKQRTIIFVYFTTIALFGYRSELVLLILQYILITNILSKD NRNPKIKRIIGYFLLVGVVCSLFYLSLGQDGEQNDSYNNMLRIINRLTIEQVEGVPYVVS ESIKNDFFPTPELEKELKAIINRIQGIKHQDLFYGERLHKQVFGDMGANFLSVTTYGAEL LVFFGFLCVFIIPLGIYIPFYLLKRMKKTHSSINCAFYSYIIMILLQYLVAGNASAFFFG PFLSVLIMCTPLILLHDTLKRLSRNENISYNCDL
Trembl: O85335
Literature
  • First Author: Nishiuchi, Y. "Structure and serologic properties of O-specific polysaccharide from Citrobacter freundii possessing cross-reactivity with Escherichia coli O157:H7". FEMS Immunol. Med. Microbiol.. 28(2). (2000), pp 163-171 PubMed: [10799808]
  • First Author: Perry, MB. "Structure of the O-chain polysaccharide of the phenol-phase soluble lipopolysaccharide of Escherichia coli 0:157:H7". Biochemistry and Cell Biology. 64(1). (1986), pp 21-28 PubMed: [3008786]
  • First Author: Vinogradov, E. "Serological cross-reaction between the lipopolysaccharide O-polysaccharaide antigens of Escherichia coli O157:H7 and strains of Citrobcter freundii and Citrobacter sedlakii". FEMS Microbiology. 190(1). (2000), pp 157-161 PubMed: [10981707]
  • First Author: Perry, MB. "Antigenic relationships of the lipopolysaccharides of Escherichia hermannii strains with those of Escherichia coli O157:H7, Brucella melitensis, and Brucella abortus". Infection and Immunity. 58(5). (1990), pp 1391-5 PubMed: [1691146]
  • First Author: Perry, MB. "The structure of the antigenic lipopolysaccharide O-chains produced by Salmonella urbana and Salmonella godesberg". Carbohydrate Research. 156. (1986), pp 107-122 PubMed: [3815404]
  • First Author: Wang, L. "Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes". Infection and Immunity. 66(8). (1998), pp 3545-51. PubMed: [9673232]