O-Antigen Description

Serogroup:O7
Serotype H:
Serotype K:1
Strain:VW187
Structure:
             aLRha(1->3)
                      |
->3)bDQui4NAc(1->2)aDMan(1->4)bDGal(1->3)aDGlcNAc(1->
Comment:- Structure [1]
- bDQui4NAc can also be called bDVioNAc, N-acetyl viosamine
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbbA
ncbi CAZy
- Reference [2]
- Putative rhamnosyl transferase according to BLAST not restricted to E. coli.
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbbB
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbbC

aDMan(1->

->4)bDGal

3 | 2
GT NameDonorAcceptorScore / e-value
O187-O187_12


105 / 2e-27
O59-WclB


90 / 4e-22
O64-O064_19


86 / 4e-21
O11-WdaF


86 / 6e-21
O36-O036_10


83 / 8e-20
O141-WfcC


82 / 2e-19
O88-O088_12


81 / 3e-19
O9-MtfB

aDMan(1->

->3)aDMan

80 / 8e-19
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbbD

bDGal(1->

->3)aDGlcNAc

4 | 1
GT NameDonorAcceptorScore / e-value
O70-O070_13


222 / 2e-72
O117-WbeD

bDGal(1->

->3)aDGalNAc

218 / 8e-71
O107-WclZ

bDGal(1->

->3)aDGalNAc

217 / 2e-70
O71-WdaK

bDGal(1->

->3)aDGalNAc

206 / 2e-66
O155-WclG


189 / 7e-60
O178-O178_09


176 / 1e-54
O153-O153_06


176 / 1e-54
O104-WbwC

bDGal(1->

->3)bDGalNAc

176 / 7e-55
O146-WbwW


172 / 2e-53
O174-WbcZ


172 / 4e-53
O112-WfeJ


172 / 2e-53
O185-O185_03


171 / 8e-53
O33-O033_09


170 / 2e-52
O37-O037_13


170 / 2e-52
O5-O05_15


167 / 3e-51
O171-O171_13


167 / 2e-51
O65-O065_13


162 / 2e-49
O160-O160_08


161 / 5e-49
O131-O131_11


153 / 6e-46
O64-O064_03


124 / 4e-35
O28-O028ab_02


112 / 2e-30
O63-WbuP


100 / 3e-26
O74-O074_09


96 / 1e-24
O114-WbuP

bDGal(1->

->3)aDGlcNAc

95 / 1e-24
O54-O054_06


94 / 4e-24
O92-O092_07


88 / 3e-22
O167-WfdH


84 / 2e-20
O127-WbiP


81 / 1e-19
O90-O090_13


81 / 1e-19
ncbi CAZy
- Reference [2][3]
NMR
1H-NMR
Solvent:
Temp:
pD:
Ref: Not Stated ppm
Comment:
13C-NMR98.85 53.50 81.90 69.40 72.50 61.65 103.90 74.30 79.35 57.30 72.20 17.80 100.60 74.30 75.15 65.90 73.30 61.50 104.70 72.00 73.30 78.00 76.15 61.35 97.30 71.15 71.65 73.30 69.90 17.80
Solvent: D2O
Temp:
pD:
Ref: Methanol 50.15 ppm
Comment: 13C-NMR [1]
Enzymes
FlippaseMNNKLARQGLLWSTIERLGTQAIQLLLMLYLGRVLGPSSFGYVGMLTLFLSLAQVLIDSG FSAALIRKSERTEKDYSTVFIFNICLSILIYIVLYCLSSYIALFYQNTSAGIFYLIFSIN RYSKWSNFNPQDSTHVDVNFKIQAKSSLIAITSSSVIAITLAALGYGVWTLVFQSLSYSV INCIVLNIYNPWYPKEKFCYETFKKLFSFSSNLLIAGVLEAFYSNIYQLIIGKFFTPQLV GQFTQANQISSVPAMTLTNIIQRVTYPLFCNIYNGKGKIDDAYLNTLKIAGVVVFPILLG IGLISKPLVSVLLGSEWKFTSDILFLLCIAFMIYPIHAININILQVHGRSDLFLRLEILK KTLMTIILVITMQININAMVIGLVLHSYLSWFLNGLFSQKVSTITIRKQLKELLPIWCLC LISGLGSNYIINEISMEPLLNIIFTILLNAIIYITLIRIMYKEIFMTIISLIKNKD
Trembl: Q9XCW5
PolymeraseMRKNFYTTPITLLILTLNIAATIVVINNSELLIGDLMGFKSETVVIIQSLIYISLSLLFL SVLYNGLEKIKPLTTKLADSNSRPIAIFLICLELLYITYVFNTGLFVAGNNERGGGVISA LFVLLNVDVLVLIFLTHVKTSKYKPIIIMLWIISFLQRGWISYFFILILISFIDRRLKNK KNWKAGVVLICFILALPFVEQIKNSIRSGERISTQLTFFDYADSFNQQFMKVVGRVQTVS HVAFITDNINQLQNLKNNGESTDFFEENFIYIIAKKLGSADDKINTPDLLARYISPSLDS SWNVNPSLIGWIFIQNDLYVLPIIWVVLLCFLFTMLSKIIANDLYAMNMRWLFWLVFLFP GWIFQFTAVIFSAFSIYFIKVHT
Trembl: Q9XCW0
Literature
  • First Author: L'vov VL. "Structural studies of the O-specific side chain of the lipopolysaccharide from Escherichia coli O:7". Carbohydrate Research. 15. (1984), pp 249-259 PubMed: [6370430]
  • First Author: Marolda, CL. "Genetic organization of the O7-specific lipopolysaccharide biosynthesis cluster of Escherichia coli VW187 (O7:K1)". Microbiology. 145. (1999), pp 2485-2495 PubMed: [10517601]
  • First Author: Brockhausen, I. "Acceptor substrate specificity of UDP-Gal: GlcNAc-R beta1,3-galactosyltransferase (WbbD) from Escherichia coli O7:K1". Glycoconjugate Journal. 25(7). (2008), pp 663-673 PubMed: [18536883]
  • First Author: L'vov VL. "Somatic antigens of Shigella and Escherichia coli. Determination of the structure of O-specific polysaccharide from Shigella boydii type 12 and its serological properties compared with the O-specific ". Bioorg. Khim. 12(9). (1986), pp 1240-1252 PubMed: [24303583]