O-Antigen Description

Serogroup:O21
Serotype H:
Serotype K:20
Strain:
Structure:
bDGal(1->4)
         |
  ->3)bDGal(1->4)bDGlc(1->3)bDGalNAc(1->
                    |
        bDGlcNAc(1->2)
Comment:- Structure [1]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclN

GT NameDonorAcceptorScore / e-value
O81-O081_07


181 / 9e-57
O83-O083_05


145 / 2e-43
O32-WfeB


137 / 2e-40
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclO
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclP

GT NameDonorAcceptorScore / e-value
O33-O033_07


107 / 2e-29
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclQ

bDGlc(1->

->3)bDGalNAc

GT NameDonorAcceptorScore / e-value
O130-WffJ


253 / 5e-85
O24-WfaM

bDGlc(1->

->3)bDGalNAc

239 / 1e-79
O134-O134_08


236 / 1e-78
O157-WbdN


234 / 1e-77
O61-WeiL


234 / 3e-78
O81-O081_11


234 / 7e-78
O46-O046_08


233 / 1e-77
O27-WejU


229 / 3e-76
O102-WelO

bDGlc(1->

->3)bDGalNAc

224 / 5e-74
O112-WfeI


185 / 9e-59
O38-O038_09


171 / 3e-53
O143-WfdV


167 / 2e-51
O140-O140_14


159 / 1e-48
O152-WfgD

bDGlc(1->

->3)bDGlcNAc

144 / 7e-43
O165-WcnW


142 / 2e-42
O173-WcmW

bDGlc(1->

->3)bDGlcNAc

124 / 3e-35
O182-O182_10


122 / 1e-34
O161-weiG

bDGlcA(1->

->3)bDGlcNAc

120 / 4e-34
O30-O030_06


119 / 2e-33
O82-O082_09


117 / 5e-33
O91-WbsA


111 / 9e-31
O56-WfaP

bDGlc(1->

->3)bDGlcNAc

109 / 5e-30
O93-O093_02


107 / 3e-29
O113-WbnA


97 / 2e-25
O128-WbsK

bDGalNAc(1->

->4)aDGal

95 / 1e-24
O79-WbdX


93 / 4e-24
O11-WdaD


82 / 4e-20
NMR
1H-NMR4.87 3.72 3.54 3.46 3.38 3.77 3.92 4.86 3.51 3.71 3.93 3.68 3.78 3.78 4.78 3.97 3.94 4.20 3.69 3.79 3.79 4.61 3.60 3.68 3.67 3.54 3.81 3.93 4.47 3.74 3.83 4.38 3.74 3.77 3.82 2.08 2.06
Solvent: D2O
Temp: 75C
pD: 6.9
Ref: TSP 0.00 ppm
Comment: 1H-NMR [1], pD=6.9
13C-NMR102.20 56.80 75.10 70.90 76.80 61.80 103.60 72.40 73.90 69.70 75.80 61.90 104.00 52.50 80.60 68.70 75.50 62.00 103.00 79.90 76.50 79.50 75.30 60.90 103.90 71.30 82.50 76.10 75.30 61.60 23.80 23.10 175.40 175.20
Solvent: D2O
Temp: 75C
pD: 6.9
Ref: Dioxane 67.4 ppm
Comment: 13C-NMR [1], pD=6.9
Enzymes
FlippaseMFTIKTVFVLRIFGAILALSTSVMISRTLNMSEAGGVFFLLSVTGIISSLSSLGQNNLILKKCASVKYAL KARGVFFLRCIKRSVISSIILSIILIPVIHYFSPQLLLRNTWCLAILLIISSSVNILLYSFLQSQGLVTF SVVLQYIFQPLLFIFFVLLIVLIKNESVLLVSLSYFFSIILIGGGGGVYSYYKFRDDFNQLETESIPFRL KELSEYFIGNSLGMLIVQSYVVLSGLLLPAADVAIIAVSDRISLVINLFAMSISTILAPKVASLYSQSKM NEIKELTKKAMFFIMIPCVLMALLFPFFSGLILSIFGVQYSNASEVLIILVMTQIINAIFCPVYVFLNMS DRQGFISKLHIYMLIPSLVITFYLTNIFGVVGIAVSKLIVVSLINIIPLIYCILLSNRVIK
Trembl: B5T081
PolymeraseMIYIIINTIVLFYMFLFNAYNRIPYGKLIIGGICVFFMTLLPAFQYGVGTDYFSYQNIYNNANELDTFYQ NKEYLFYGYMRLYQLTGWGFKGFIALTALLQSLLVFVIVFQLWKNYGYSLVLVFFLFWVVTNLFHTQMNI IRASFSIYLFAISILYKFRGKLLLSFILMVAALGFHRSALIGFCFLVIPDKMYFFACKHAFKIYVLTFLL FLFSYLQQIIYYIVQNLFPYYSHYLSSFDESSVSIMNVLTKMYWIPFNLLFLILLRLKVFVIKDNERKLI GMWALTVNVYLLMLSFDFISRVNYYFVIFYIIPIIYVIKYTVKNRYFVCLYLSLLYCFIPYLLKVILFPI AEFYYKSYLF
Trembl: B5T085
Literature
  • First Author: Staaf, M. "Structural elucidation of the O-antigenic polysaccharides from Escherichia coli O21 and the enteroaggregative Escherichia coli strain 105". European Journal of Biochemistry. 266(1). (1999), pp 241-245 PubMed: [10542071]
  • First Author: Ren, Y. "Characterization of Escherichia coli O3 and O21 O antigen gene clusters and development of serogroup-specific PCR assays". Journal of Microbiological Methods. 75(2). (2008), pp 329-334 PubMed: [18700154]