O-Antigen Description

Serogroup:	O66
Serotype H:	25
Serotype K:
Strain:
Structure:	->2)bDGlc3Ac(1->3)aLTal6d(1->3)aDGlcNAc(1->2)bDMan(1->3)aDGlcNAc(1->
Comment:	- Structure [1]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfaA

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfaB

->2)bDGlc

GT Name	Donor	Acceptor	Score / e-value
O144-O144_03			145 / 4e-42
O152-WfgE			143 / 5e-41
O56-WfaQ			128 / 2e-35
O24-WfaO	aDGlc(1->	->2)bDGlc	126 / 7e-35
O29-WffY			125 / 2e-34
O169-O169_04			122 / 1e-33
O183-wbaT			120 / 9e-33
O81-O081_10			112 / 4e-30
O102-WelN	aDGal(1->	->6)bDGlc	105 / 2e-27
O130-WffI			104 / 4e-27
O173-WcmX	aDGlc(1->	->2)bDGlc	103 / 9e-27
O18-wcxD			102 / 1e-26
O27-WejX			96 / 3e-24
O187-O187_10			90 / 3e-22
O85-WfbQ			87 / 3e-21
O65-O065_10			82 / 1e-19
O110-O110_03			81 / 4e-19

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfaC

GT Name	Donor	Acceptor	Score / e-value
O55-WbgP			101 / 5e-27
O55-WbgP			101 / 6e-27
O112-WfeF			90 / 1e-22
O102-WelO	bDGlc(1->	->3)bDGalNAc	90 / 7e-23
O115-WegI			89 / 3e-22
O165-WcnW			83 / 2e-20
O30-O030_06			83 / 2e-20
O24-WfaM	bDGlc(1->	->3)bDGalNAc	82 / 2e-20
O113-WbnA			82 / 3e-20
O143-WfdV			81 / 1e-19
O46-O046_08			81 / 5e-20
O134-O134_08			81 / 6e-20

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfaE

GT Name	Donor	Acceptor	Score / e-value
O109-weiT	aL6dTal(1->	->3)bDGlcNAc	194 / 3e-60

ncbi CAZy

- Reference [2]

NMR
1H-NMR		5.02 3.89 3.87 3.64 4.24 1.21 5.11 3.98 3.88 3.56 4.02 3.82 3.82 4.73 3.92 3.76 3.71 3.40 3.94 3.80 5.43 4.06 3.95 3.56 4.10 3.80 3.80 4.74 3.52 3.59 3.47 3.43 3.77 3.72
Solvent:	D2O
Temp:	90C
pD:
Ref:	Acetone 2.225 ppm
Comment:	1H-NMR [1]
13C-NMR		102.80 70.90 76.50 71.70 68.90 16.70 99.60 55.00 81.00 69.30 73.10 61.70 101.40 78.10 74.90 68.20 78.10 62.00 97.20 53.90 81.30 69.30 73.10 61.60 104.80 75.70 77.40 67.00 76.80 61.60 23.30 174.70 23.30 174.40 21.45 173.90
Solvent:	D2O
Temp:	90C
pD:
Ref:	Acetone 31.45 ppm
Comment:	13C-NMR [1]

Non-Sugar Components
- Acetate

Enzymes
Flippase		MNYLVNRKDIIWGYLSQFLNISSSLLLLPFILKCLNEEEIGLWYVFVAMAGIIQLLEFGLLPTISRFISY VYSGALNLEYGKIPKCNIGDFNKELLNDVVVTCRCIYFKIALFALVGILILGNAYIYTLSTSINKYYILC CWSVYGLSICIQLYFGYYNAILKGRGDQTLLNKIIVVAKLTLLLISIPLLIAGCGLVSLAVGAVISVIID RILIRKYVYSQESGLIRNLEKSNKTVSLKKVIWQSARDMGVVQLGNYLSVRAGVLIVSSFVNLDAAASYG LTVQVTMVIVIIASMFFGLNIPRITSEQAHENMLVVKNLMKKSLLIANCIYIFAALILVAIGDDILAFLT KNTTLLPKELLILYLVTALFEMNYSICTSYLTTRNEVIFIKSMLYTGVAIVLFSFISTYIAEMGALGVIL SQLLMQSLYNNWHWPLKVYQELKND
Trembl:	Q1AL85
Polymerase		MKNKISFYIAYIYIAPIILSLYYISTTNSLLGDFRTYSYKLEWESSLLFLILYFIPLIIIFCVVKMTGKI KVDTFESIKLEKFAFYSIIITGLITLIFGANAIGHPAQTGIAGVIIKIASKLNPLVLLPLLTFTNVSVRK FFFCAAVVIFYGYMQQSLQGIYISVLCAGAFFLLKVRLNGAIFILMLLSPIIFLGPLLDIITHLYTIRNE IRGVSFSVEEIFSLALGRISTTSSLIYTISNSFNTTGVSDYFVFGIVIERLIGVHILDTVSPSQVFNTYI LGANADYSIFMGLAGFLYFIKDFTCSVYCKCCVFDLGTDSYILGANADYSIFMGLAGFFVFLLKTSPAVF IVNAACLILVLILIYLLIPVNNVKYRTSVFFLIMYLPFLSFDIWEISIVFQTIIIWRVFLLLSKISFGLK
Trembl:	Q1AL83

Literature
First Author: Jann, B. "NMR investigation of the 6-deoxy-L-talose-containing O45, O45-related (O45rel), and O66 polysaccharides of Escherichia coli". Carbohydrate Research. 278(1). (1995), pp 155-165 PubMed: [8536267]
First Author: Cheng, J. "Genetic characterization of the Escherichia coli O66 antigen and functional identification of its wzy gene". Methods Microbiology. 45(1). (2007), pp 69-74. PubMed: [17342059]